Ligation problems

[Francois Nantel]

Dear bionetters,

I presently have big ligation problems but that was expected. I need to
put a 3kb insert into a 3.2 kb vector. Oh, did I tell you it is a blunt
ligation :-0

I tried treating the vector with alkaline phosphatase but the ligation
effeciency is so low I didn't get anything.

If anybody have a knock-out technique for hard to do ligation, I would be
very grateful.


Francois Nantel
IGBMC
nantel at titus.u-strasbg.fr