QIAquick Spin Columns
pekrappu at sara.cc.utu.fi
Fri Mar 10 02:44:31 EST 1995
In article <Pine.3.89.9412051637.A14125-0100000 at bongo.cc.utexas.edu>, hempel at BONGO.CC.UTEXAS.EDU ("Alice L. Hempel") writes:
> Does anyone have a recipe for a solubilization buffer (QX1) for use with
> the Qiagen QUIquick Spin Columns for DNA extraction from agarose gels?
> We always run out of this before any of the other components of the kit.
> This usually gives us good yields of PCR product for direct sequencing,
> with less work than the glass milk methods, but it is frustrating to run
> out of one thing long before any of the others.
> Alice Hempel
> hempel at bongo.cc.utexas.edu
Perharps you have already noticed that QIAGEN Inc. sells both QX1 and
PE buffers individually. QX1 buffer comes in 300 or 100 ml
bottles. I do not know the price of these products but I
assume it is reasonable.
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