lambda insert isolation

Cheung Cho Yue, M.D. ccy at po.cwru.edu
Sat Mar 11 17:51:06 EST 1995


We have isolated a clone from a lambda gt10 library, but EcoRI did
not release the large fragment, although a smaller 5' fragment was
released.  PCR has not been helpful in getting out a single large 
band.  Are there alternative useful enzyme sites in gt10?

Thanks.

Cyue at mhnet.mhmc.org




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