Phenol distillation

Shahram Mori smori at nmsu.edu
Sat Mar 11 23:14:53 EST 1995


Jim Owens (jow at helix.nih.gov) wrote:
: In article <3jpms8$j6i at owl.und.ac.za> Roy Dace, dace at shrike.und.ac.za
: writes:
: >Can any of you suggest whether distillation of new AR phenol should be
: >necessary?

: Equilibrate with water or buffer.  If the phenol is colorless, with no
: reddish tinge, it should be OK to use as is.

: Good luck,

: Jim Owens
That's right. I equilibrate with buffer containing Tris-HCL (7.4), NaCl, 
and EDTA.  I do not do any boiling. Though this is a pain in the butt 
too, it's much safer.
Cheers
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003





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