Amplicon's purification.

SPAGNOL at PLAUTO.CSATA.IT SPAGNOL at PLAUTO.CSATA.IT
Sun Mar 12 04:36:42 EST 1995


I read that a couple of spins in centricon 100 devices are sufficient to
purify your amplification product. What about the Taq, which remains in the
retentate? Does it interfere with RE digestion or with direct sequencing?
Thanks a lot in advance from a PCR's beginner.



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