COS-1 cells wanted
bhandary at wccf.mit.edu
Mon Mar 13 19:09:00 EST 1995
In article <3jhi33$95h at amcnix.amc.uva.nl>, seppen at amc.uva.nl writes...
>Until now we have always used COS-7 cells for transient expression.
>Unfortunately we are now only allowed to use them under special safety
>conditions. COS-1 cells do not have a complete integration of SV-40 virus so we
>can use these in our lab. Does somebody know were we can get these cells??
>Thanks in advance
>SEPPEN at AMC.UVA.NL
I would go with getting them from ATCC (American Type Culture Collection); the
shipping charges will probably set you back a bit, but the stuff will be
certified. Perhaps there is a similar organization nearer to you. BTW, in
using both cos-7 and cos-1 with sv40 ori based plasmids, I've found stronger
signals using the cos-1. I think that the cos-1 is suppose to have higher
levels of T-antigen.
No one said anything specific to us about using cos-7; is this a recent
development in your institute. At present, we operate under bl2 rules (since
some of our vectors have > 70% of the full SV40 genome). Perhaps this
automatically covers for cos-7 anyway.
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