SYBR Green

Jfloring at Jfloring at
Mon Mar 13 17:40:18 EST 1995

 Sandy and all:
As a real SYBR green fan, I'd like to respond to the end of Sandy's question:

"I would also be interested to know whether you can add it to the gel
before running or if it is necessary to stain afterward.
YES!! Add SYBR green to your gel and buffer, at about 0.1-0.5x the
concentration recommended for post-staining.  You won't have to stain, or
wait for destaining.  It's hard to go back to EtBr once you've tried it.
   Jeanne  Loring

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