Cracked SSCP gels
rdewar at julian.uwo.ca
Mon Mar 13 23:15:17 EST 1995
Can anyone tell me why my polyacrylamide gels are cracking while drying?
I have been running SSCP gels on 10% polyacrylamide gels, some containing
10% glycerol and some without. As with most problems, things have been
going well for some time, and then the gels started to crack when I
removed them from the gel dryer.
Is it too much heat, too much vacuum or too much time? I dry
them for 1 1/2 hours at 65 C.
Thanks for any suggestions.
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