Request for Site directed mutagenesis Protocols (PCR)
hooperkp at aol.com
Mon Mar 13 22:48:03 EST 1995
I modified the method of Horton, et al. (1990) Biotechniques 8, 528-535.
Rather than join two unlike cDNA's like the method described in the paper,
I use the general principles to generate mutants in my target cDNA. So
far, I have not failed to create my specific mutant. A lab next door uses
Pfu polymerase for these procedures. I have found that Taq works fine.
If you need more specifics, e-mail me.
Kyle P. Hooper, Ph.D.
UT Southwestern Medical Center
Dept. of Microbiology
Dallas, TX 75225-9048
HooperKP at AOL.COM
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