Request for Site directed mutagenesis Protocols (PCR)

Hooperkp hooperkp at aol.com
Mon Mar 13 22:48:03 EST 1995


Michael,

I modified the method of Horton, et al. (1990) Biotechniques 8, 528-535.
Rather than join two unlike cDNA's like the method described in the paper,
I use the general principles to generate mutants in my target cDNA.  So
far, I have not failed to create my specific mutant.  A lab next door uses
Pfu polymerase for these procedures.  I have found that Taq works fine. 
If you need more specifics, e-mail me.

Kyle P. Hooper, Ph.D.
UT Southwestern Medical Center
Dept. of Microbiology
Dallas, TX  75225-9048
HooperKP at AOL.COM 



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