TA cloning -Promega

Edward Wang ez022056 at rocky.ucdavis.edu
Mon Mar 13 21:10:42 EST 1995


I have been using the TA cloning Kit (p-Gem T system) by Promega.  
Talking to others, I found out that the T overhangs on the PCR products 
falls off after a certain amount of time, therefore decreasing the 
cloning efficiency in a TA system.  The best way was to PCR, and then 
immediately clone into vector.  Has anyone else heard anyting like this?


--
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Edward H. Wang                 *
Staff Resch. Assc.             *
Dept. Internal Med. -Nephrology*
UC Davis                       *
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