Want PCR to incorporate 32P label

kang at msvax.mssm.edu kang at msvax.mssm.edu
Fri Mar 10 17:21:50 EST 1995


In article <9503101947.AA03207 at seralph6.essex.ac.uk>, jone3 at essex.ac.uk (PAUL JONES) writes:
>Has anyone had success with incorporation of 32P dNTP into an extending strand
>in PCR for northern/ southern hybridisation?  I would have thought is would 
>give 
>higher signal / noise ratio than probes produced by random hexamer method 
>and a method of choice for probes <200 bp.
>
>Hope there is opinion out there.
>


	
I tried this method without any success.
So far for me, random priming is the best choice.

Chulho Kang



More information about the Methods mailing list