Linear Polyacrylamide

(Ian Ridgers) ir at mailserver.nhm.ac.uk
Mon Mar 13 10:43:01 EST 1995


On 10 Mar 1995 18:11:53 GMT,
  Louis Hom writes:

>I'm trying to do a zymographic experiment, where I copolymerize a protein
>substrate into my polyacrylamide gel, then run a cell extract to look at the
>MW of a protease that digests the substrate (it leaves a hole).
> Anyway, my problem is that my substrate is only about 40 kD, so it
>migrates a significant distance during the electrophoresis.  I've heard that
>you can conjugate the substrate to linear polyacrylamide so that it will stay
>in place (the conjugated complex has a MW ca. 10^6).  Until I can get my hands
>on some pre-made linear polyacrylamide, can anyone suggest a way to prepare
>my own? 
>
>P.S. I don't have tons of substrate, which is why I'm co-polymerizing the
>substrate in the gel instead of diffusing it into the gel post-electrophoresis.


Try the method taken from the paper 'Etanol precipitation of DNA with 
linear polyacrylamide as carrier'

Nucleic Acids Research, Vol.18, No.2
Ian Ridgers
The Natural History Museum
Cromwell Rd.                       
London. SW7 5BD              

E-mail ir at nhm.ac.uk
Tel. 0171 938 9297

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