can formamide harbour RNases?

Shahram Mori smori at
Mon Mar 13 02:31:58 EST 1995

Dear netters,
I was wondering if formamide can harbour any RNases. We were de-ionizing 
our formamide using Sigma's Amberlite Mesh16-50. We used the newly 
de-ionized material for our RNA denaturing buffer and everything 
including the ladder was degraded. I used a 2 month old denaturing buffer 
that included ( Formamide, formaldehyde, MOPS, Depc H2O) and it worked 
(so much for needing to make this stuff FRESH). 
Thanks for your input.

Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||

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