Gel box less than 1 dollar

Nsubuan Johnson 0355515 at SHU.SACREDHEART.EDU
Mon Mar 13 10:06:22 EST 1995


YOU ARE A GREAT ONE!!!

On 10 Mar 1995 kang at msvax.mssm.edu wrote:

> Hi fellows;
> 
> Once I posted that I can make a gel tank which cost less than
> 1 dollar. I was so surprised by the responses from  all over the
> world. At first time, my greedy mind told me to file a patent
> and make some fortune. However, I thought again and changed
> my mind. Since it can help many people who does not have big
> grant, I just decided let the idea go out.
> 
> I REALLY HOPE THIS SMALL IDEA CONTRIBUTE TO THE DEVELOPMENT
> OF SCIENCE EVEN A LITTLE BIT.
> 
> My invention is simple.
> 
> USE CARBON ROD AS AN ELECTRODE INSTEAD OF PLATINUM WIRE. I
> found the carbon core of pencil is a great electrode. If you
> flame your pencil with the burner, only the core remains and
> it is perfect for using. Although this electrode does not
> last forever, you can use more than hundred times.
> 
> Getting box is also very simple. A guy mentioned a device
> using Tupper (spelling?) ware. I don't against that idea but
> I am using cheaper box. I found that BOX COME WITH PIPETTE
> TIP is very good for gel box. You don't have to pay for the
> box.
> 
> How to make? It is also very simple.
> 
> Make  two holes little bigger than the carbon core diameter
> at opposite side of the bottom corners and pork in your
> electrode through the hole. You can make hole easily by using
> hot (flame with burner) Pasteur pipette. Electrode can be
> fixed to the box using several kind of plastic glues. I am
> using silicon glue but thinking epoxy glue will be the best.
> The empty space of the hole also should be filled with glue.
> The carbon electrode should be a little longer than the width
> of the box so 0.5 cm of the electrode remains out side of the
> box.
> 
> That's it.
> 
> However, here is one problem. How can you connect power? It
> is also simple to figure out.
> 
> If you go electric shop like Radio Shock, you can find
> alligator clip. Clip your electrode and connect it.
> Because this device has a protruding electrode outside of the
> box you should be careful for electric shock. I recommend
> all the metal part of the clip and electrode be covered with
> a vinyl tubing.
> 
> The beauties of this device is simplicity and economy.
> 
> However, real beauties are not those. Here are real beauties.
> 
> 
> 1. Running gel is much faster; If you apply the same voltage
> you can run gel twice faster than submarine gel with mounted
> bed. Usually I finish gel running within 20 min if it is not
> a complicate DNA.
> 
> 2. Only very small amount of buffer (I am using TAE) is
> required and the buffer can be reused.--Dream of the environmentalist like me.
> 
> 3. Resolution of the gel is incredibly clean.
> 
> 4. Running of DNA band is very even, so you don't have a
> skewed band patterns.
> 
> 5. DNA purified from this gel is pure enough for ligation.
> 
> 6. You can make double deck or triple deck gel without
> problem.
> 
> 
> 
> I mentioned miracle gel box in my previous posting. However,
> I think that device is a little dangerous if you are not
> careful. So I decided not to reveal that one. I also
> mentioned Fax sending or TIFF file transfer. However, I guess
> whoever has common sense (I think all of you) can figure it
> out without any problem. So, I am deleting that part.
> Sorry.
> 
> 
> Cheers,
> 
> Please tell me if you have another improvement
> 
> 
> Chulho Kang
> Wizard of TM (transgenic mice)
> 
>       
> 
> 



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