HELP ME!! (lig/trans: UV and CIP) -- repeat message -Reply

[David Osterbur]

Dear Heidi,
Short wave UV can cause major problems with subcloning.  If you do not have access to long wave, a good alternative is to run two lanes of your sample.  Stain one, cut it out and use the remaining gel slice as a template for cutting out your band of interest from the non-UV, non-ethidium-exposed DNA.
Good luck!