Phenol distillation

4700gbera at 4700gbera at
Tue Mar 14 10:16:53 EST 1995

In article <3jtsft$6f9 at dns1.NMSU.Edu>, smori at (Shahram Mori) writes:
>Jim Owens (jow at wrote:
>: In article <3jpms8$j6i at> Roy Dace, dace at
>: writes:
>: >Can any of you suggest whether distillation of new AR phenol should be
>: >necessary?
>: Equilibrate with water or buffer.  If the phenol is colorless, with no
>: reddish tinge, it should be OK to use as is.

>That's right. I equilibrate with buffer containing Tris-HCL (7.4), NaCl, 
>and EDTA.  I do not do any boiling. Though this is a pain in the butt 
>too, it's much safer.

Or, better yet,if you don't need tons of the stuff, buy some pre-equilibrated
phenol. I bought some phenol/chloroform/isoamyl alc. from USB, and it's worth
every penny. It's expensive, so if you need a lot of it, it'll run into  a lot
of money. But I have a 400 ml bottle that's going to last me until I get of my

Gina Berardesco
4700gbera at
University of Massachusetts, Boston


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