Want PCR to incorporate 32P label

Zal Suldan Z-Suldan at ski.mskcc.org
Tue Mar 14 08:35:08 EST 1995


> >Has anyone had success with incorporation of 32P dNTP into an extending strand
> >in PCR for northern/ southern hybridisation?  I would have thought is would 
> >give 
> >higher signal / noise ratio than probes produced by random hexamer method 
> >and a method of choice for probes <200 bp.


What about end-labelling the primer/s?? Or won't that be hot enough?

Zal

All opinions are mine and not of MSKCC



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