Want PCR to incorporate 32P label
Z-Suldan at ski.mskcc.org
Tue Mar 14 08:35:08 EST 1995
> >Has anyone had success with incorporation of 32P dNTP into an extending strand
> >in PCR for northern/ southern hybridisation? I would have thought is would
> >higher signal / noise ratio than probes produced by random hexamer method
> >and a method of choice for probes <200 bp.
What about end-labelling the primer/s?? Or won't that be hot enough?
All opinions are mine and not of MSKCC
More information about the Methods