Two hybrid Q.

Rafael Maldonado rafael at corona
Tue Mar 14 15:22:27 EST 1995

Hi folks!

I'm trying the two hybrid method for detecting interacting proteins in yeast.
I have seen that the proteins to be tested are disposed in the C-terminal 
of the gal4 protein domains (both). I mean, the fusions are always in the 
N*gal4domain-yourfavoriteprotein*C direction.

Because I'm interested in the interaction with the N-terminal end of my 
protein, can I use the fusion in the other way, N*Myprotein-gal4domain*C?

Have anyone tried that? Do you think it would work?




Rafael Maldonado			"You gave to a boy the job  
Department of Human Genetics		 of a man. He's dead."
University of Utah			
Salt Lake City, Utah 84112. USA.	                     John Wayne
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