COS-1 cells wanted

Randy Haun rhaun at nih.gov
Wed Mar 15 09:58:31 EST 1995


In article <1995Mar14.223310.17489 at alw.nih.gov> bernard at elsie.nci.nih.gov (Bernard Murray) writes:
>Newsgroups: bionet.molbio.methds-reagnts
>Path: nih-csl!bernard
>From: bernard at elsie.nci.nih.gov (Bernard Murray)
>Subject: Re: COS-1 cells wanted
>Message-ID: <1995Mar14.223310.17489 at alw.nih.gov>
>Summary: What is the difference (*facts* please)?
>Keywords: COS-1 cells
>Sender: postman at alw.nih.gov (AMDS Postmaster)
>Nntp-Posting-Host: elsie.nci.nih.gov
>Organization: National Institutes of Health
>References: <3jhi33$95h at amcnix.amc.uva.nl>
>Date: Tue, 14 Mar 1995 22:33:10 GMT
>
>I was interested to read of the posts concerning the use of COS-1 vs COS-7
>cells.  A colleague of mine who "was there when COS cells were made" assures
>me that, at least at the outset, there was only one line (COS-1) and that
>COS-7 originated as a typo brought about by someone who used a large serif
>on their figure 1.  I realise that the line(s) have been about for some
>time and so artificial differences could arise so, what is the popular
>or personal perception of differences between COS-1 and "COS-7"?
>Does anyone have any hard data (rather than "ease of transfection" or
>growth rates etc.)?  Maybe this is a case of literary, rather than genetic,
>mutation.... ;-)
>			Bernard
>
>Bernard Murray, Ph.D.
>bernard at elsie.nci.nih.gov  (National Cancer Institute, NIH, Bethesda MD, USA)
>

The reference I have always used for the "COS" cell lines is:

Gluzman Y., "SV40-Transformed Simian Cells Support the Replication of Early
SV40 Mutants", Cell 23 (1981) 175-182

In this paper the author reports the establishment of three "COS" cell
lines, namely COS-1, COS-3, and COS-7, that were "obtained by transformation
of CV-1 cells with an origin-defective mutant SV40".

As all three cell lines appear to have been established after transformation
with the same origin-defective mutant SV40, it is not clear why a laboratory
would be prohibited from using one particular cell line and not one of the
others (as indicated in the original post).

Randy Haun
NIH/NHLBI



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