ELECTROPORATION PARAMETERS FOR LETTUCE PROTOPLASTS

NICOL,NATHALIE,MS XPWQ000 at MUSICB.MCGILL.CA
Wed Mar 15 11:41:52 EST 1995


HI!  I NEED TO USE ELECTROPORATION ON LETTUCE PROTOPLASTS AND I AM NOT
SURE WHAT PARAMETERS WOULD BE THE MOST APPROPRIATE.  OUR UNIT IS A
BIORAD, THE CUVETTES ARE 0.4 CM.  IF YOU HAVE ANY SUGGESTION ON WHAT
VOLTAGE, CAPACITANCE, ETC. I SHOULD USE, PLEASE ANSWER A.S.A.P.  YOUR
HELP WOULD BE MUCH APPRECIATED.  THANK YOU!




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