Transfection efficiency

Ian A. York york at mbcrr.dfci.harvard.edu
Thu Mar 16 17:08:12 EST 1995


In article <3ka6a0$32e at mark.ucdavis.edu> dobates at ucdavis.edu (Dave Bates) writes:
>I've been wondering about how people measure their transfection
>efficiency. A lot of messages here, and in papers talk about

I have an easy out - I measure transfection efficiency in transients, in 
COS, by surface expression in FACS.  Relatively objective.  It seems to 
me that "transfection efficiency" should mean percentage of cells which 
express the gene, so you pretty much have to do a cell count.  I agree 
with your concern about subjective levels.  

Ian
-- 
Ian York   (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-4328     Fax  (617)-632-2627




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