apoptosis detection: problems with test kits

[Inst. for Biochemistry]

Hello world


I'm trying to detect apoptosis by the Terminale-Transferase-
Reaction.
For detection I'm using biotinylated dUTP and streptavidin-
peroxidase with DAB (Diaminobenzidine).

Using flow-cytometry I know the samples are apoptotic, but I can't see 
it with the dye. But in the future I' ll be forced to use it, as I'm 
going to work with lower cell-densities.

Is anybody working with apop-kits or something similar and can give me
any hints, especially concerning a non fluorescent dye.



Thanks for your answers!

                        dirk kaiser

Technische Hochschule Darmstadt
Institut fuer Biochemie
Petersenstrasse 22
64287 Darmstadt
Tel.: 06151/165157
e-mail: dh7y at pop.th-darmstadt.de