kferri at email.unc.edu
Fri Mar 17 02:33:42 EST 1995
Hello! Please pardon the simple question, but I just started my first
job as tech. and therefore haven't learned everything yet.
Homogenized some tissue, isolated and purified the RNA, checked OD's, and
ran on a gel to check RNA integrity. For 1 sample, the gel lane was
blank (not even smeared, which would indicate denaturation). I ran 3
gels to be sure the problem of possible RNA omission to gel lane was
addressed. Still no run, even after adding extra RNA to lane. The  of
the sample (I checked it twice) was 17% lower the second time, but acc.
to my boss, still not low enough to not show up.
Any ideas? Thanks in advance!
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