"missing" RNA

Karen Ferri kferri at email.unc.edu
Fri Mar 17 02:33:42 EST 1995

Hello!  Please pardon the simple question, but I just started my first 
job as tech. and therefore haven't learned everything yet.

Homogenized some tissue, isolated and purified the RNA, checked OD's, and 
ran on a gel to check RNA integrity.  For 1 sample, the gel lane was 
blank (not even smeared, which would indicate denaturation).  I ran 3 
gels to be sure the problem of possible RNA omission to gel lane was 
addressed.  Still no run, even after adding extra RNA to lane.  The [] of 
the sample (I checked it twice) was 17% lower the second time, but acc. 
to my boss, still not low enough to not show up.

Any ideas?  Thanks in advance!


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