Pischia expression

PWHoward pwhoward at aol.com
Fri Mar 17 01:36:14 EST 1995


Pischia has produced a full length transcription factor for me that E.
coli just couldn't hack.  It was poly His tagged and was purified easily
in one step.  When using Ni++ chelate resin I have found that binding your
protein to the resin in 0.6 M ammonium sulfate substantially increases
yield.  The high salt can be washed of the resin just prior to elution
with 0.25 M imidazole.



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