why fix sequencing gel?

Kirk Bartholomew kbarthol at moose.uvm.edu
Thu Mar 16 14:07:08 EST 1995

I have had veery poor results exposeing my gels without fixing.  However 
I believe this is due to the fact that I use 35S not 32P.  The lab down 
the hall uses 32P, does not fix, and their gels look fine.  The answer: 
if you are using 32P it is not necessary to fix.

More information about the Methods mailing list