Rf: How "random" are random primers?
smori at nmsu.edu
Sat Mar 11 23:07:07 EST 1995
LOGAND (logand at msdos.montpellier.inra.fr) wrote:
: Allen Black wrote:
: >A friend of mine asked an interesting question. What is the G-C content of a
: >random hexamer? Before you flame, I know that it would be 50% if it is
: >truly random sequence. The more relevant question is perhaps is a random
: >hexamer truly random or is the G-C content decreased to increase the annealing
: >temperature of the hexamer. My friend had no luck with the local tech-reps.
: >Can someone out there help my friend out?
I thought all random hexamer meant was a sample that included ALL
possible nucleotide sequences. Random hexamers are done in a reaction
mixture that is 35-40C so I don't know how much importance annealing
temperature will make in this case.
Shahram Mori _/\_
Program in Molecular Biology _\ /_
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
More information about the Methods