Multiple IgG bands in reduced SDS-PAGE

Dayanidhi Raman maraman at ksu.ksu.edu
Thu Mar 16 20:31:19 EST 1995


I use affinity purified antibodies (polyclonal) & I am getting multiple IgG bands on reduced SDS-PAGE. I use 2-ME and my sample buffer is very fresh. If I do western blots on my protein I get multiple bands because these H-L bands bind secondary antibody & answer in ECL detection. Need help!.



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