In Situ Hybridization

[Brian Halloran]

Dear Netters:	
  I'm wondering if anyone has experience using the in situ hybr	
  protocol of Brigati et al.  According to their article in J. 
  Clin. Lab Analysis (7:247-51, 1993), they can do colormetric
  ISH in 90 min.  Of course they use a microprobe staining
  station from Fischer Scientific (cost ~$1500-2000).  Does this
  protocol really work?  Any personal experience feedback will be
  appreciated.  Thanks in advance.  Please e-mail replies to :

       bghallor at unmcvm.unmc.edu

       Brian G. Halloran