Autoclaving Dextran Sulfate?

Stephen R. Lasky, Ph.D. Stephen_Lasky at brown.edu
Thu Mar 16 10:23:48 EST 1995


In article <3k6qje$olc at susscsc1.rdg.ac.uk>, szsbejos at reading.ac.uk (Dr
Joseph Behnam) wrote:

> In article <3joqj0$lbg at server.st.usm.edu>, sywang at whale.st.usm.edu
(Shiao Y. Wang) says:
> >
> >Carlisle Landel (landel at helios) wrote:
> >: We use dextran sulfate in our hyb solutions.  We keep a 33% stock of
> >: the stuff, which it turns out started growing some kind of bug.  Can
> >: you autoclave dextran sufate with impunity?  (Filtering it is an
> >: exercise in patience in the *extreme*.)
> 
> We use 50% Dextran Sulfate for Southern/signal amplification.
> Simply add 0.2% Sodium Azide for perservation. It work fine for more
than one year!
> Regards
> Joseph Behnam
> Developmental Biology Research Group
> School of Animal & Microbial Sciences
> The University of Reading

A long time ago I autoclaved 50% DexSulfate (so long ago that the details
are not clear anymore).  But, you can do it although you should be
prepared for your solution to be a gel at high temp.  It goes back into
solution when it cools.

SRL

-- 
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Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
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