Molar extinction coefficient of a protein at 280 nm.

Ralf Morgenstern Ralf.Morgenstern at imm.ki.se
Fri Mar 17 02:27:41 EST 1995


In article <3k9cmu$ngb at hermes.fundp.ac.be>, madi at biocell.fundp.ac.be (Marc
DIEU) wrote:

> Is it possible to determine the molar extinction coefficient at 280 nm
> of a given protein (epsilon 280 nm prot.) simply by additionning the
> individual molar extinction coefficients of the different aromatic
> residues which compose the sequence of the protein ? Is it correct or
> not ? Is there any literature available about it ? Is the absorbance of
> a residue influenced by its environment or by its position in the
> three-dimentional structure of the protein ? The non-aromatic residues,
> do they also contribute to some extent to the absorbance of the protein
> at 280 nm ? What is the value of the molar extinction coefficient at
> 280 nm of Trp, Tyr, Phe and His ? 

Check out "Calculation of protein extinction coefficients from amino acid
sequence data, Anal. Biochem. 182, 319-326 (1989) by Gill and von Hippel.

-- 
Ralf Morgenstern, IMM, Karolinska Institutet, Box 210, S-17177, SWEDEN
Tlf. +46-8-7287574, Fax, +46-8-334467, E-mail: ralf.morgenstern at imm.ki.se



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