RNAse protection assay FIXED!

Katherine Schaefer ks3f+ at andrew.cmu.edu
Fri Mar 17 11:30:27 EST 1995


    Thanks to everyone who responded to my question about the random
degradation of (presumably) protected fragments.  The problem was that
the RNAse A was not being totally inactivated before the RNAs came in
contact with denaturing buffer.  Addition of a proteinase K step or
multiple phenol/CHCl3 extractions solved the problem.
        Thanks again!
            Katherine Schaefer CMU Biology



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