RNAse protection assay FIXED!
ks3f+ at andrew.cmu.edu
Fri Mar 17 11:30:27 EST 1995
Thanks to everyone who responded to my question about the random
degradation of (presumably) protected fragments. The problem was that
the RNAse A was not being totally inactivated before the RNAs came in
contact with denaturing buffer. Addition of a proteinase K step or
multiple phenol/CHCl3 extractions solved the problem.
Katherine Schaefer CMU Biology
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