why fix sequencing gel?
g9013118 at mcmail.cis.mcmaster.ca
Fri Mar 17 11:25:54 EST 1995
In article <1995Mar16.190708.22339 at emba.uvm.edu>,
Kirk Bartholomew <kbarthol at moose.uvm.edu> wrote:
>I have had veery poor results exposeing my gels without fixing. However
>I believe this is due to the fact that I use 35S not 32P. The lab down
>the hall uses 32P, does not fix, and their gels look fine. The answer:
>if you are using 32P it is not necessary to fix.
If you use 35S it is not necessary to fix, either. All we use is 35S in
sequencing, we don't fix our gels, and we get great results.
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