why fix sequencing gel?

K. Legate g9013118 at mcmail.cis.mcmaster.ca
Fri Mar 17 11:25:54 EST 1995


In article <1995Mar16.190708.22339 at emba.uvm.edu>,
Kirk Bartholomew <kbarthol at moose.uvm.edu> wrote:
>I have had veery poor results exposeing my gels without fixing.  However 
>I believe this is due to the fact that I use 35S not 32P.  The lab down 
>the hall uses 32P, does not fix, and their gels look fine.  The answer: 
>if you are using 32P it is not necessary to fix.
>
If you use 35S it is not necessary to fix, either. All we use is 35S in 
sequencing, we don't fix our gels, and we get great results.



kyle





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