DNase treatment of RNA

Jillian Johnston johnstoj at acs.ucalgary.ca
Fri Mar 17 11:11:31 EST 1995


Why not just autoclave the DNase buffer?  I rarely use
DEPC-treated water and have found that autoclaving all solutions
is sufficient to remove RNases.  We do a lot of in vitro
transcription and RNA protection assays and in the past 4 years I
have used DEPC once. Occassional problems we've had are seldom
found to be with the lab-prepared solutions.
Good luck.
Jillian Johnston



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