Competent Yeast Cells

Zal Suldan Z-Suldan at ski.mskcc.org
Fri Mar 17 08:21:21 EST 1995


In article <MICDAT.28.2F690BC5 at ccs1.cc.monash.edu.au>
MICDAT at ccs1.cc.monash.edu.au (Dirk Truman) writes:

> G'day
> 
> I am looking for a protocol to prepapre competent yeast cells that can be 
> stored long term at -70.   Does such a thing exist or am I just being hopeful?
> 
> Thanks,
> 
> 
> Dirk Truman      Department of Microbiology     Telephone  61 3 905 4830
>                               Monash University                               


electrocompetents work great in my hands, are very convenient to make,
and can be stored at -70. Follow procedure in red book (chapter 13 --
Sac. Cerevesiae). Bonus: making electrocomps' and transforming is
easier than doing it the old Li+ way. Good luck.


Zal

Z-Suldan at ski.mskcc.org



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