immunofluorescence background

Dave Gilbert gilbertd at VAX.CS.HSCSYR.EDU
Fri Mar 17 06:54:53 EST 1995

Does anyone have any tricks to reduce apparently non-specific association
of polyclonal antibodies.  We have tried blocking steps with serum,
however, our immunofluorescent signals with antibody are still only
marginally above signals obtained with pre-immune serum.


Dave Gilbert
Department of Biochemistry and Molecular Biology
SUNY Health Science Center
750 E. Adams St.
Syracuse, N.Y. 13210
Tel:(315) 464-8723
FAX:(315) 464-8750

More information about the Methods mailing list