salger at wap18.zi.biologie.uni-muenchen.de
Fri Mar 17 17:42:42 EST 1995
wmbonner at helix.nih.gov wrote:
: I've had good luck with the pCR-script kit from Stratagene. It uses a
: rare 8-base cutter (I forget
: which) to recut the vector each time it closes without the insert.
: No need to dephos. Good as long
: as your frag does not have the 8-base cutter site.
The same does of course work with every enzyme, provided that its site
is unique in the vector and doesn't cut the insert.
Klaus Salger phone : ++49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG MacWilliams e-mail: salger at zi.biologie.uni-muenchen.de
More information about the Methods