Ligation problems

Klaus Salger salger at wap18.zi.biologie.uni-muenchen.de
Fri Mar 17 17:42:42 EST 1995


wmbonner at helix.nih.gov wrote:
<snip>
: I've had good luck with the pCR-script kit from Stratagene.  It uses a
: rare 8-base cutter (I forget 
: which) to recut the vector each time it closes without the insert.
:  No need to dephos. Good as long 
: as your frag does not have the 8-base cutter site.
: -Bill

The same does of course work with every enzyme, provided that its site
is unique in the vector and doesn't cut the insert.

Cheers
  Klaus

--
Klaus Salger                phone : ++49 (0)89 5902 -502
Zoologisches Institut       FAX   :                 -450
AG MacWilliams              e-mail: salger at zi.biologie.uni-muenchen.de
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80333 Muenchen
Germany



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