RT-PCR using S1 Nuclease

Petra M. Jakobs, Ph.D. jakobsp at ohsu.edu
Fri Mar 17 17:32:22 EST 1995


From: Philip S. Ruban  Sahaayar at ohsu.edu

I am trying to do RT-PCR, using S1 nuclease. Basically, I first extract
my RNA and throw the probe of my interest into the total RNA. Then cut
 with S1 nuclease and
amplyfy the hybrid, using PCR. 
Could anyone know the protocol for this method?
If so, please me kno as soon as you can.
Thanks.



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