Sticky DNA after Hind III-Why?

LOGAND logand at msdos.montpellier.inra.fr
Fri Mar 17 14:34:47 EST 1995


Does anyone know/or can provide a educated guess as to why genomic DNA, 
when digested with Hind III, smears down the side of the tube after 
precipitation (standard sodium acetate/ethanol) and spining, while other 
REs give nice pellets? This is not a problem, just curiosity.
Is it because of the buffer, the enzyme, interactions with the salt?

All answers gratefully read.

Thanks

David C. Logan
INRA-ENSA(M)-CNRS
Montpellier
FRANCE

Logand at msdos.montpellier.inra.fr




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