DTT in in vitro transcription Rx

John Fox J.R.Fox at shu.ac.uk
Sat Mar 18 15:12:13 EST 1995


In article <3kfb0s$1o8 at lyra.csx.cam.ac.uk>, flh at mole.bio.cam.ac.uk (Frances Hannan (Zoology)) says:
>
>li_je at a1.mscf.upenn.edu (jie li) writes:
>
>>  Both in vitro transcription and common PCR rx include 1mM DTT in their
>>reaction mix.  I am told that DTT is a reducing reagent, but I still don't
>>know its function in that case.  Could someone tell me?
>
>The DTT in in vitro transcription is necessary for the RNAse Inhibitor to 
>work.
>
>-- 
>Frances Hannan                                       
>BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
>Phone (0223)336663, FAX (0223)461954                 
>flh at mole.bio.cam.ac.uk                               

In my experience DTT can be present as a stabilizing factor for the polymerase
enzyme.

John



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