SYBR and gel staining

Ian Clements ianc at efn.org
Fri Mar 17 00:13:12 EST 1995


To use SYBR G 1 correctly post-staining is the most sensitive approach.

You can also add the dye to the DNA sample in the loading buffer.  The
final dye concentration must be 1:10000 or you will get retardation of 
the DNA possibly even reverse migration.

You can add the dye to the gel during casting but you'll use a great deal 
more dye and your DNA will migrate strangely.



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