TA cloning ratio

[Edward Wang]

I am currently using the Promega p-GemT system and having lots of 
problems with it.  I am not getting colony growth.  My guess is that the 
insert to vector ratio is too low (They recommend 1:1 ratio).  
Has anyone who used this kit any 
suggestions?  Right now, my controls are not working either (a insert 
which they provide).  Also, is it myth or fact that for cloning, one 
should use fresh PCR product because the overhangs fall off?  Any 
suggestions would be appreciated.

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Edward H. Wang                 *
Staff Resch. Assc.             *
Dept. Internal Med. -Nephrology*
UC Davis                       *
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