DTT in in vitro transcription Rx

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Sat Mar 18 14:07:08 EST 1995


li_je at a1.mscf.upenn.edu (jie li) writes:

>  Both in vitro transcription and common PCR rx include 1mM DTT in their
>reaction mix.  I am told that DTT is a reducing reagent, but I still don't
>know its function in that case.  Could someone tell me?

The DTT in in vitro transcription is necessary for the RNAse Inhibitor to 
work.

-- 
Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk                               



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