DTT in in vitro transcription Rx

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Sat Mar 18 14:07:08 EST 1995

li_je at a1.mscf.upenn.edu (jie li) writes:

>  Both in vitro transcription and common PCR rx include 1mM DTT in their
>reaction mix.  I am told that DTT is a reducing reagent, but I still don't
>know its function in that case.  Could someone tell me?

The DTT in in vitro transcription is necessary for the RNAse Inhibitor to 

Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk                               

More information about the Methods mailing list