2-D Electrophoresis

[P. Friedl]

In article <ayele_bandele-0803951610010001 at mac101490.med.cornell.edu>, ayele_bandele at qmcumc.mail.cornell.edu (ya-ya) says:
>
>>  This is my first time posting so bear with me.
>>  I recently started running 2-D gels using the Bio-Rad system.
>>  My first question is what can be done to prevent the horizon-
>>  tal lines in the gel?
>>  I am solubilizing in 2% NP-40,4% ampholyte, 9.5M Urea and 100mM DTT.
>>  Next question, when running an 8mm X 10mm 2nd dimension gel, is it 
>>  normal for it to run for an hour >  and 45 minutes.
>>  Finally, I recently purchased gelcode color Silver Stain; but the 
>>  only colors I see are brown and yellow spots on an amber background. Normal?
>>
>>  Does this group have a FAQ listing? if so how do I find it?
>>
>>  Hope someone can help me :->
>>  TIA
>>  Ayele

	In our case the horizontal line was caused by Tris in the 
electrode buffer; using another lot abolished the problem