di58 at pop.th-darmstadt.de
Fri Mar 17 05:01:23 EST 1995
In article <ayele_bandele-0803951610010001 at mac101490.med.cornell.edu>, ayele_bandele at qmcumc.mail.cornell.edu (ya-ya) says:
>> This is my first time posting so bear with me.
>> I recently started running 2-D gels using the Bio-Rad system.
>> My first question is what can be done to prevent the horizon-
>> tal lines in the gel?
>> I am solubilizing in 2% NP-40,4% ampholyte, 9.5M Urea and 100mM DTT.
>> Next question, when running an 8mm X 10mm 2nd dimension gel, is it
>> normal for it to run for an hour > and 45 minutes.
>> Finally, I recently purchased gelcode color Silver Stain; but the
>> only colors I see are brown and yellow spots on an amber background. Normal?
>> Does this group have a FAQ listing? if so how do I find it?
>> Hope someone can help me :->
In our case the horizontal line was caused by Tris in the
electrode buffer; using another lot abolished the problem
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