immunofluorescence background

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Sat Mar 18 18:34:00 EST 1995


I have had good luck with SuperBlock from Pierce supplemented
with 0.05% Tween 20 to remove non-specific interaction.
Remember what may seem like non-specific binding can be specific
- try using Fab or Fab2 fragments
Serum is usually the aboslute pits in both situations, it can at
times ENHANCE non specific interaction.



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