shake after electroporation?

Qunfeng Dong qfdong at iastate.edu
Sun Mar 19 22:04:10 EST 1995


Hi,there:

   I used to shake cells after electroporation at 37C for 1 hrs, somebody
told me it'd better not shake,just plate cells! the reason is that if cells 
grow quickly, the plasmid DNA could be kicked out of cells. I never heard
this before, is it right?

Qunfeng.



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