Church for Northern blots?

Glen Shearer gshearer at whale.st.usm.edu
Sun Mar 19 19:30:49 EST 1995


Manuel Maria SIMON <Simon at mail.boku.ac.at> wrote:
>
> Hi
> 
> Did anyone out there ever (successfully) tried church also 
> for northern blots? Are there special tricks to get it work like 
> on southerns?
[[snip]]

We use a slight modification of it for both southerns & northerns
and it works fine (0.5 M sodium phosphate, pH ~7.0-7.2, 5% w/v SDS).

Regards,
Glen





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