Curing E.coli plasmids
rafael at corona.med.utah.edu
Mon Mar 20 14:33:46 EST 1995
On Mon, 20 Mar 1995, Duncan Clark wrote:
> Hi Folks,
> Has anyone got a good high efficiency method for curing both colE1
> replicon based plasmids (pBR322 deriv.) and p15A replicon based
> plasmids (pACYC series). EtBr, Acridine Orange, electroporation
> (if plasmids can go in they can also come out!) etc.
You can use coumermycin. It's a inhibitor of the DNA gyrase and the
pasmids are lost at sub-lethal concentrations. I used 5
ug/ml and it works well with pBR322-derivated plasmid. You can use it for
others replicons as well.
The refrence is:
Danielvskaya, ON and Gragerov, AI (1980). Curing of Escherichia coli K12
plasmids by coumermycin. MGG 178, 233-235.
You can find coumermycin at Sigma (C-9270). Not relation with the company!
Rafael Maldonado "You gave to a boy the job
Department of Human Genetics of a man. He's dead."
University of Utah
Salt Lake City, Utah 84112. USA. John Wayne
Rafael at genetics.med.utah.edu
Rafael at corona.med.utah.edu "No por mucho madrugar, amanece
Tlf: 801-581-4429 mas temprano"
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