Curing E.coli plasmids

[Rafael Maldonado]

On Mon, 20 Mar 1995, Duncan Clark wrote:

> Hi Folks,
> 
> Has anyone got a good high efficiency method for curing both colE1 
> replicon based plasmids (pBR322 deriv.) and p15A replicon based 
> plasmids (pACYC series). EtBr, Acridine Orange, electroporation 
> (if plasmids can go in they can also come out!) etc.

You can use coumermycin. It's a inhibitor of the DNA gyrase and the 
pasmids are lost at sub-lethal concentrations. I used 5 
ug/ml and it works well with pBR322-derivated plasmid. You can use it for 
others replicons as well.

The refrence is:
Danielvskaya, ON and Gragerov, AI (1980). Curing of Escherichia coli K12 
plasmids by coumermycin. MGG 178, 233-235.

You can find coumermycin at Sigma (C-9270). Not relation with the company!


Rafa



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Rafael Maldonado			"You gave to a boy the job  
Department of Human Genetics		 of a man. He's dead."
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