pGex-cryptic promoters in GST?
dalel at u.washington.edu
Mon Mar 20 17:27:31 EST 1995
Has anyone experienced constitutive expression of genes cloned into
Pharmacia's pGex plasmids? We put an assayable reporter (beta-gal) into
the MCS of pgex and get lots of activity (1/10 induced w.t.) in uninduced
cultures. When we immuno-precipitate with anti-beta gal and run on a gel, we
see a heavy band at about the right place for a GST-beta-gal fusion.??
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