How long would it take to clone a gene from a plasmid?

Pete Muriana MURIANAP at FOODSCI.PURDUE.EDU
Mon Mar 20 17:07:03 EST 1995


Netters,
I wonder if someone would be so kind as to indicate how long it would take a 
reasonably skilled person to confirm the presence of a gene on a suspected 
plasmid with an oligo probe by Southern blot analysis and clone a gene/gene 
fragment from a plasmid (given no unusual problems) given the following:

     1. A similar gene from a related bacterium was already sequenced and the 
         sequence was available,

     2. A pair of primer sequences, either for PCR or for use as oligo probes, 
         were determined on day 1 using a Primer software program.

    3. The plasmid, of about 20-kb, was already available (i.e., purified) and 
        previously correlated with loss of phenotypic expression by 
        curing.

Thank-you,
Peter

p.s. - This is not for myself but to illustrate a point to someone else. 
         Thanks.
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*  Peter M. Muriana, Ph.D.             317-494-8284   TEL            *
*  Dept. of Food Science               317-494-7953   FAX            *
*  Purdue University                   murianap at foodsci.purdue.edu   *
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