How long would it take to clone a gene from a plasmid?

[Pete Muriana]

Netters,
I wonder if someone would be so kind as to indicate how long it would take a 
reasonably skilled person to confirm the presence of a gene on a suspected 
plasmid with an oligo probe by Southern blot analysis and clone a gene/gene 
fragment from a plasmid (given no unusual problems) given the following:

     1. A similar gene from a related bacterium was already sequenced and the 
         sequence was available,

     2. A pair of primer sequences, either for PCR or for use as oligo probes, 
         were determined on day 1 using a Primer software program.

    3. The plasmid, of about 20-kb, was already available (i.e., purified) and 
        previously correlated with loss of phenotypic expression by 
        curing.

Thank-you,
Peter

p.s. - This is not for myself but to illustrate a point to someone else. 
         Thanks.
**********************************************************************
*  Peter M. Muriana, Ph.D.             317-494-8284   TEL            *
*  Dept. of Food Science               317-494-7953   FAX            *
*  Purdue University                   murianap at foodsci.purdue.edu   *
*  Smith Hall                                                        *
*  W. Lafayette, IN  47907-1160                                      *
**********************************************************************