smori at nmsu.edu
Sun Mar 19 18:33:44 EST 1995
Fiberman (william at neuro.usc.edu) wrote:
: I want to do immunocytochmistry with sections of rabbit brains. What would
: be a bigger problem: if the primary antibody is generated in rabbits or
: if the secondary antibody is generated in rabbits? Assuming both are
: polyclonal antibodies. Are there tricks to reduce background if one of
: the antibodies is from rabbit?
I would try affinity Purifying my antibodies using sephadex columns.
Shahram Mori _/\_
Program in Molecular Biology _\ /_
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
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